Coding

Part:BBa_K209466:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI B-C part, G2A


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
    Illegal PstI site found at 214
    Illegal PstI site found at 568
    Illegal PstI site found at 1039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
    Illegal PstI site found at 214
    Illegal PstI site found at 568
    Illegal PstI site found at 1039
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
    Illegal PstI site found at 214
    Illegal PstI site found at 568
    Illegal PstI site found at 1039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
    Illegal PstI site found at 214
    Illegal PstI site found at 568
    Illegal PstI site found at 1039
    Illegal NgoMIV site found at 171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 550


Design Notes

Cloned into pCR2.1.

Source

Plasmid obtained from [http://www.cdna.org/G-Protein-Coupled-Receptors-c8.html Missouri S&T cDNA Resource Center].

References